Cells were also labeled with MitoTracker™ Red CMXRos (Mitochondria, green) and Draq 5 (Nucleus, magenta). Bufalin induces mitochondrial dysfunction and promotes apoptosis of ... MitoView™ Fix 640 staining retains better specificity after PFA fixation compare to MitoTracker® Deep Red FM. spectral-imaging-with-versachrome - Semrock Mitotracker staining - Alsford Lab time: 1.8s. Comparison of Electronic Volume and Forward Scatter Principles ... - Wiley KIT - Botanisches Institut - Nick-Labor A basic principle in fluorescence microscopy is the highly specific visualization of cellular components with the help of a fluorescent agent. (1958) Principles of biological microtechnique (Methuen, London). 2XRos, and MitoTracker® Deep Red are retained in the mitochondria after fixation. Plate reader, microscope, flow cytometer. Frontiers | MitoTracker Deep Red (MTDR) Is a Metabolic Inhibitor for ... In principle, there are several factors that . In order to simultaneously excite all the . The other two, MitoTracker-Red and MitoTracker-Orange, showed multiple fluorescence lifetime populations in their phasor plots (Supporting Figure S1). Cell viability assay guide | Abcam Channel Slides . For imaging of MitoTracker Red CMXRos, 561 nm excitation wavelength (0.3 power setting) was used and emission was collected at 595 nm by a GaAsP PMT detector (gain of PMT HV 30). Figure 1 - The Basic Principle of STORM Imaging. MitoTracker Red: 578 nm: 599 nm: MitoTracker Red, MeOH: 578 nm: 599 nm: X-Rhod-1 Ca 2+ 580 nm: 602 nm: Alexa Fluor 568 antibody conjugate pH 7.2: 579 nm: 603 nm: Alexa 568: 576 nm: 603 nm: 5-ROX pH 7.0: Extracellular ATP is increased by release of ATP-loaded ... - Theranostics 6. Monitor mitochondrial membrane potential with MitoView . PDF LysoTracker and LysoSensor Probes - Thermo Fisher Scientific Thanks to Dr. Lemasters et al., they reviewed the chemistry and questioned that MitoTracker Orange . MitoTracker® Green FM | Cell Signaling Technology The Principle of Lattice Light Sheet Microscopy . Isolated myofibers were stained with mitotracker deep red (M2246, Thermo Fisher) and NucBlue (R37605, Thermo Fisher) in DMEM for 30 min. SYBR Gold dye enables preferential labelling of mitochondrial ... - PLOS